Amicon ultra 15 manual

Amicon Ultra-2 Centrifugal Filter Devices 9 How to Use Amicon Ultra-2 Centrifugal Filter Devices 1. Insert the Amicon Ultra-2 device into the filtrate collection tube. 2. Add up to 2 mL of sample to the device and cover with concentrate collection tube. 3. Place filter device into the centrifuge rotor and counterbalance with a similar device. 4. Centrifugal filter units, Amicon® Ultra Filters Centrifugal Filters. These centrifugal filter units can be used for a number of applications: concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms; purification of macromolecular components found in tissue culture extracts or cell. Amicon® Ultra centrifugal filters are constructed with a styrene/butadiene housing, polypropylene centrifuge tube and a rigid polyethytlene cap and liner. % integrity tested for reliable performance. The vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5-minutes).

Amicon Ultra-4 Amicon Ultra Centrifugal Filter Devices Concentration Factor (X) Spin Time (min) 0 20 10 15 30 40 60 80 Spin Time vs Concentration Factor Performance shows results in Amicon Ultra 4 mL device with a 4 mL starting sample of cytochrome c, 12, daltons ( mg/mL) at 3 spin times (10, 15, and 30 minutes). Filtrate. using the Amicon® Ultra mL device. Samples were processed according to the Amicon® Pro user manual1. The Amicon® Ultra mL device was inserted into a mL microcentrifuge tube. µL of the eluted fraction was added and centrifuged at 14, g for 15 min. This process was repeated twice more to concentrate the entire sample. Millipore’s Amicon® Ultra centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices.

Centrifuge device at 4, × g for 15 minutes in a swinging-bucket rotor. 3. Recover the labeled antibody from the Amicon® Ultra device by reverse spin. 4. Amicon Ultra-4 Series laboratory equipment pdf manual download. or fixed-angle rotor with wells/carriers that can accommodate 17 mm × mm 15 mL. Amicon® Ultra 15 ml. MWCO. Stock code. Product description. UFC Amicon® Ultra 15ml 3k 8pk. 3 KDa UFC | Amicon® Ultra 15ml 3k 24pk.